Restriction digests of the clone give the following sizes (kb): BglI--2.2, 1.1; BglII--3.3; EcoRI--2.7, 0.65; HindIII--3.0, 0.3; PvuII--2.3, 1.0. The insert contains the following restriction sites (nt position according to the published sequence): HindIII--4007, 4338; BglII--4285. Detects the following genomic EcoRI fragments (kb): human--11; hamster--4.9, 1.5; mouse--5.7, 2.4. Contains the 27 C-terminal codons and the 3' untranslated region extending from the EcoRI site at nt 3759 of the published sequence to an EcoRI linker added approximately 70 bp beyond the HindIII site at nt 4338. |
