Insert contains the following sites separated by (bp)(approx): BamHI- 540- PvuII- 180- BstEII- 110- BamHI. Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 0.77; PvuII--3.1, 0.61; BstEII--3.8; HindIII/EcoRI--2.9, 0.84; HindIII--3.8. Detects a single copy sequence at high stringency, but detects pseudogenes at low stringency. pBamtheta is the 830 bp BamHI fragment of calpha3'pSVED of E. Whitelaw. (calpha3'pSVED is the 2.5 kb EcoRI/HindIII fragment of calpha3'Bg cloned into the ScaI site of pSVED.). |
