| Insert |
DNA: genomic Insert lengths(kb): 11.0 Gene product: DNA Segment, single copy [D17S122] Alleles: A1, A1, A2, A3, B1, A2, B2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--9.4, 4.3; EcoRI--11.0, 2.7; BamHI--6.0, 3.2, 2.4, 2.1; PstI--2.5 (doublet), 2.4, 2.0, 1.1 (doublet), 0.7, 0.6, 0.4; SalI--13.7. This is a subclone of VAW409 (p07486). This probe may contain repetitive sequences. The 2.8/2.7 kb constant bands are the same polymorphism detected by pVAW409R3 (ATCC 61476, 61477) but hybridization is light and the 1.9 kb constant band blocks reading the 1.9 kb allele. The 2.7 band obscures reading the A2 allele. The combined heterozygosity of both MspI polymorphisms at D17S122 detected by pVAW409R1 (ATCC 61474, 61475) and pVAW409R3 (ATCC 61476, 61477) is 72%. |
| References |
Wright EC, et al. A genetic map of human chromosome 17p. Genomics 7: 103-109, 1990. PubMed: 2335351
Lebo RV, et al. Multicolor in situ hybridization and linkage analysis order Charcot-Marie-Tooth type 1 (CMT1A) gene-region markers. Am. J. Hum. Genet. 50: 42-55, 1992. PubMed: 1729894
Lupski JR, et al. DNA duplication associated with Charcot-Marie-Tooth disease type 1A. Cell 66: 219-232, 1991. PubMed: 1677316
David F Barker, personal communication
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