| 產(chǎn)品名稱 |
Y-223a |
| 商品貨號(hào) |
B241716 |
| Designations |
Y-223a |
| Species |
Homo sapiens, human |
| Depositors |
U Muller, Institute of Human Genetics |
| Vector |
Construct size (kb): 5.20
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 |
| Insert |
DNA: genomic
Insert lengths(kb): 0.75
Genome: Homo sapiens
Gene symbol: DYZ5
Genomic copy number: repetitive
Gene name: DNA Segment, repetitive
Contains complete coding sequence?: U
Chromosome: Y; Localization: Y p
Tissue: 49,XXXXY cell line
Type of DNA: genomic
Insert end: HindIII
Insert end: HindIII |
| Insert Size (kb) |
0.75 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--5.2; HindIII--4.4, 0.76; NotI--uncut; EcoRI--5.2; PstI--5.2. Y-223a maps closer to the centromere than Y-280. Y-223a hybridizes to DNA in 9/16 46,XX males and a female with a dicentric Y chromosome. |
| References |
Muller U, et al. Deletion mapping of the testis determining locus with DNA probes in 46,XX males and in 46,XY and 46,X,dic(Y) females. Nucleic Acids Res. 14: 6489-6505, 1986. PubMed: 3748818
Gaunt SJ. Mapping of testis-determining locus on Yp by the molecular genetic analysis of XX males and XY females. Development 101: 51-58, 1987. PubMed: 88211484
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