This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level
1
Host
Distribution host: Escherichia coli 294; GMBH K-12 294
Distribution host: Escherichia coli 294; GMBH K-12 294
pGH6 is available in a bacteria culture as ATCC 31539 or as purified plasmid DNA as ATCC 40012.
Expression vector. The EcoRI site is downstream of the lacUV5 promoters.
This was constructed by inserting a 285 bp EcoRI fragment from pKB268 (containing 2 95 bp lac UV5 promoters separated by heterologous DNA) into the EcoRI site of pBR322. The EcoRI site distal to the tetR gene was destroyed.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Goeddel DV, Heyneker HL. Method of constructing a replicable cloning vehicle having quasi-synthetic genes. US Patent 4,342,832 dated Aug 3 1982
