-20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Introduction
Cell Line Description:
HCC1937BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from the same patient as HCC1937 (a primary breast ductal carcinoma cell line, ATCC CRL-2336). BRCA1 analysis revealed that this cell line is heterozygous for the BRCA1 5382C mutation, whereas the primary tumor cell line is homozygous for the same mutation.? DNA isolated from the cell line HCC1937 is available as CRL-2336D.
Quality Control Specifications
> 100 kb in size by agarose gel electrophoresis
No RNA detected in the agarose gel
A260/A280 ratio ≥ 1.8
Tested and verified for PCR amplification and restriction digestion.
