Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 0.72; PvuII--2.3, 0.62, 0.43; KpnI--3.4; HindIII--3.4; BamHI--3.4. The insert contains the following restriction sites (approximate kb from the 5' end): StuI--0.26; PvuII--0.54; KpnI--0.66. Nucleotides 1-711 of the insert correspond to nt 3916-4626 of the sequence record. This insert is derived entirely from the 3' untranslated region. One of 4 clones (pI7-11, pF5-1, pA2-10, pA2-13, ATCC 65988-65995) that together contain the complete coding sequence. Cloned in the antisense direction. |
