Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.7; HindIII--5.7; BamHI--4.6, 1.1; PstI--5.7; EcoRI/HindIII--3.3, 2.4. The insert contains the following restriction sites (nucleotide position according to the published sequence): PstI--156; KpnI--322; BamHI--1062; KpnI--1174; HindIII--2353. Derived from the 9 or 11 kb mRNA and not the 3 or 5 kb mRNA. Contains an open reading frame of 2205 nucleotides and includes about 520 nucleotides of 3' untranslated sequence. The insert lacks sequences encoding the amino-terminal 4 short consensus repeats. Detects mRNA species of 3, 5, 9 and 11 kb and genomic EcoRI fragments of 12 kb. |
