produces protein 1-aminocyclopropane-1-carboxylate deaminase
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Shipping Information
Distributed: freeze-dried
Preceptrol®
no
Type Strain
no
Comments
Growth in M9 plus 0.05 mM ACC or co-expression of ACC synthase complement the Ile auotrophy.
E. coli host expressing 1-aminocyclopropane-1-carboxylic acid (ACC) deaminse from the recA promoter.
Strain was derived from JHM-544 (ATCC 12911), an Ile auxotroph, by chromosomal integration of the ACC deaminase using cre-lox-mediated recombination.
Medium
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
Name of Depositor
A Theologis
Special Collection
NCRR Contract
References
Tarun AS, et al. Random Mutagenesis of 1-aminocyclopropane-1-carboxylate synthase: a key enzyme in ethylene biosynthesis. Proc. Natl. Acad. Sci. USA 95: 9796-9801, 1998. PubMed: 9707555
Klee HJ, et al. Control of ethylene synthesis by expression of a bacterial enzyme in transgenic tomato plants. Plant Cell 3: 1187-1193, 1991. PubMed: 1821764
Hasan N, et al. Escherichia coli genome targeting, I. Cre-lox-mediated in vitro generation of ori- plasmids and their in vivo chromosomal integration and retrieval. Gene 150: 51-56, 1994. PubMed: 7959062
