| Insert |
DNA: genomic Insert lengths(kb): 4.400000095367432 Tissue: fetal liver Gene product: argininosuccinate synthetase(arginosuccinate synthetase 1) [ASS] Alleles: A1, A1, A1, A2, A2, A2 |
| Comments |
When excised from pBR322 with EcoRI + HindIII, insert and vector are about the same size. Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.4; EcoRI--10.0; BamHI--10.0; BamHI/EcoRI--5.0, 4.0; PvuII--4.4. Insert contains exons 11 and 12 as well as some intron sequence of the argininosuccinate synthetase gene. The population studied was CEPH (Centre d'Etude du Polymorphisme Humain) families. Enzyme(s) not detecting polymorphism: EcoRV, KpnI, ScaI, SphI, SstI, BanI, BclI, BglI, BglII, EcoRI, HincII, BanII, Fnu4HI, MboI, RsaI, TaqI, HindIII, PssI, PvuII, StuI, XbaI. |
| References |
Northrup H, et al. Multilocus linkage analysis with the human argininosuccinate synthetase gene. Genomics 5: 442-444, 1989. PubMed: 2575581
Hope Northrup, personal communication
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