| 產品名稱 |
phi6-3 |
| 商品貨號 |
B233454 |
| Designations |
phi6-3 |
| Species |
Homo sapiens, human |
| Depositors |
M Litt |
| Applications |
Standard hybridization and washing conditions can be used, with a final wash in 0.1X SSC/0.1% SDS at 68C. The constant band at 2.5 obscures the B2 allele when the entire phage is used as probe. Because the 2 loci are in total disequilibrium, use of locus A alone provides all available information. |
| Vector |
Construct size (kb): 44.0 |
| Insert |
DNA: genomic Insert lengths(kb): 14.5 Gene product: DNA Segment, single copy [D11S85] Alleles: A1, B2, A2, B1 |
| Insert Size (kb) |
14.5 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried bacteria-free lysate |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--25.0, 12.5, 2.0; SalI--19.5, 14.5, 10.0. phi6-3 contains repeated sequences and must be prehybridized with total human DNA before it will detect single copy bands. Standard hybridization and washing conditions can be used, with a final wash in 0.1X SSC/0.1% SDS at 68C. MspI detects 2 diallelic RFLPs. The constant band at 2.5 obscures the B2 allele when the entire phage is used as probe. Because the 2 loci are in total disequilibrium, use of locus A alone provides all available information. |
| References |
Nakamura Y, et al. Localization of the genetic defect in multiple endocrine neoplasia type I within a small region of chromosome 11. Am. J. Hum. Genet. 44: 751-755, 1989. PubMed: 2565085
Misra BC, Srivatsan ES. Localization of HeLa cell tumor-suppressor gene to the long arm of chromosome II. Am. J. Hum. Genet. 45: 565-577, 1989. PubMed: 2577469
Maslen CL, et al. Seven polymorphic loci mapping to human chromosomal region 11q22-qter. Genomics 2: 66-75, 1988. PubMed: 2898428
Michael Litt, personal communication
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