Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.0, 0.5; BamHI--4.5; PstI--4.0, 0.5; BglII--4.5; EcoRI/HindIII--2.9, 1.0, 0.5. The insert contains the following restriction sites (nt from the 5' end): StuI--40; BglII--87; SmaI--151; EcoRI--472; PstI--99, 554. Contains the complete coding sequence, a 5' untranslated region of 102 bp, and a 3' untranslated region of 870 bp. Constructed with the 5' end of the cDNA closer to the T7 promoter site. |
