| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--23.5, 13.5, 10.8; HindIII--25.3, 6.4, 5.6, 4.7, 2.4, 2.1; BamHI--25.3, 13.5, 9.8; PstI--15.4, 12.3, 3.2, 2.7, 2.1, 1.8, 1.3, 1.1, 0.92 + smaller; SalI--45. This insert contains part of exon 5, intron 5, exon 6, and 3' flanking sequences. There are 2 EcoRI (intron 4, 3' flanking), 1 BamHI (intron 4), and 2 HindIII (3' flanking) sites in the insert. |
