Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 0.8; HindIII--3.8; PstI--2.3, 1.6; ClaI--3.8; XhoI--2.8, 0.9. Exact restriction sizes are as follows: BamHI--2.98 kb, 0.86 kb; HindIII--3.84 kb; PstI--2.27 kb, 1.57 kb; ClaI--3.84 kb; XhoI--2.92 kb, 0.92 kb. The PstI site given in the paper at the 5' end does not exist. The plasmid contains the PstI site in bla and a PstI site in the insert. Interleukin 4 and interleukin 5 are encoded in the same 345 kb NotI fragment. Contains about 100 bp of poly(A). The insert contains the complete coding sequence and includes NheI, EcoRV, PstI, PvuII, BglI, and EcoRI sites. |
