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BL29-169

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編號:B230068

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產品名稱	BL29-169
商品貨號	B230068
Designations	BL29-169
GenBank Number	T27246 T27247
Species	Homo sapiens, human
Depositors	R Sanders
Vector	Construct size (kb): 3.849999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ', <- primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, <- promoter: lac, <- primer site: M13 reverse, <- replicon: pMB1 marker(s): ampR, <- Cross references:
Insert	DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene name: DNA Segment, numerous copies, expressed Contains complete coding sequence?: U Chromosome: 7 Genome: Homo sapiens Gene name: DNA Segment, numerous copies, expressed Contains complete coding sequence?: U Chromosome: 3 Tissue: BL29 cell line Disease: Burkitt's lymphoma Type of DNA: cDNA Insert end: Modification: EcoRI linker Insert end: Modification: EcoRI linker Insert size (kb): 0.95 Cross references: DNA Seq. Acc.: T27246; EST: EST169; Primer: T7 DNA Seq. Acc.: T27247; EST: EST169R; Primer: T3 Insert lengths(kb): 0.949999988079071 Tissue: BL29 cell line Burkitt's lymphoma Disease: Burkitt's lymphoma Gene product: DNA Segment, numerous copies, expressed
Insert Size (kb)	0.950
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments	Restriction digests of the clone give the following sizes (kb): BamHI--4.0; EcoRI--3.1, 0.9; HindIII--4.0; PstI--4.0; SacI--4.0.

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