| Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 3.407, 5.813, 2.064, 3.028, 2.505, 3.971, 0.941, 1.169, 1.031, 1.203, 0.520, 2.072, 1.938, 3.727, 0.899, 0.564, 1.207, 0.473. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.40, 0.49, 0.55, 0.93, 0.96, 1.1, 1.2, 2.0, 2.2, 2.6, 3.3, 3.6, 3.9, 4.2, 6.3. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70193, 70882, 70883, 70895, 71124. |
