| 產品名稱 | BHK-21 [C-13] |
|---|---|
| 商品貨號 | B164033 |
| Organism | Mesocricetus auratus, hamster, Syrian golden |
| Tissue | kidney |
| Cell Type | fibroblast |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Age | 1 day old newborn |
| Applications | The World Organization for Animal Health (OIE) uses these cells for routine diagnosis of rabies. This cell line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.g., Factor VIII, see ATCC CRL-8544). |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | Chromosome Frequency Distribution 50 Cells: 2n = 44. This is a pseudodiploid line with the tetraploidy occurring at 4%. The karyotype is 44,XY,-6,-15,6q+,15q+ in a majority of cells analyzed. The markers 6q+ and 15q+ occurred in most cells. An occasional monosomic or trisomic condition for a normal chromosome was also detected. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Images |  |
| Derivation | The parent line of BHK-21(C-13) was derived from baby hamster kidneys of five unsexed, 1-day-old hamsters in March, 1961, by I.A. Macpherson and M.G.P. Stoker. Following 84 days of continuous cultivation, interrupted only by an 8-day preservation by freezing, clone 13 was initiated by single-cell isolation. |
| Virus Susceptibility | Human adenovirus 25
Reovirus 3 Vesicular stomatitis virus Human poliovirus 2 |
| Comments | This cell line is reverse transcriptase negative. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: 1 to 2 times per week |
| Cryopreservation | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C |
| Name of Depositor | I Macpherson |
| Deposited As | Mesocricetus auratus |
| Year of Origin | March, 1961 |
| References | Drayna D, et al. Genetic mapping and diagnosis of haemophilia A achieved through a BclI polymorphism in the factor VIII gene. Nature 314: 738-740, 1985. PubMed: 2986011 Kazazian HH Jr., et al. Restriction site polymorphism in the phosphoglycerate kinase gene on the X chromosome. Hum. Genet. 66: 217-219, 1984. PubMed: 6325324 Macpherson I, Stoker M. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence. Virology 16: 147-151, 1962. PubMed: 14468055 Macpherson, et al. Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. Virology 14: 359-370, 1961. Macpherson I. Characteristics of a hamster cell clone transformed by polyoma virus. J. Natl. Cancer Inst. 30: 795-815, 1963. Deleersnyder V, et al. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71: 697-704, 1997. PubMed: 8985401 Yang TT, et al. Quantification of gene expression with a secreted alkaline phosphatase reporter system. BioTechniques 23: 1110-1114, 1997. PubMed: 9421645 Hussain MA, et al. POU domain transcription factor brain 4 confers pancreatic alpha-cell-specific expression of the proglucagon gene through interaction with a novel proximal promoter G1 element. Mol. Cell. Biol. 17: 7186-7194, 1997. PubMed: 9372951 You M, et al. ch-IAp1, a member of the inhibitor-of-apoptosis protein family, is a mediator of the antiapoptotic activity of the v-Rel oncoprotein. Mol. Cell. Biol. 17: 7328-7341, 1997. PubMed: 9372964 Jelachich ML, Lipton HL. Theiler's murine encephalomyelitis virus kills restrictive but not permissive cells by apoptosis. J. Virol. 70: 6856-6861, 1996. PubMed: 8794327 Schnell MJ, et al. The minimal conserved transcription stop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus. J. Virol. 70: 2318-2323, 1996. PubMed: 8642658 Chang YE, et al. Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1. J. Virol. 70: 3938-3946, 1996. PubMed: 8648731 Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS ISO 10993.5-2002. Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999. |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
